- JOB
- Poland
- EXPIRES SOON
Job Information
- Organisation/Company
- Institute of Biochemistry and Biophysics Polish Academy of Sciences
- Research Field
- Biological sciences
- Researcher Profile
- First Stage Researcher (R1)
- Positions
- PhD Positions
- Country
- Poland
- Application Deadline
- Type of Contract
- Temporary
- Job Status
- Full-time
- Hours Per Week
- 40
- Offer Starting Date
- Is the job funded through the EU Research Framework Programme?
- Not funded by a EU programme
- Is the Job related to staff position within a Research Infrastructure?
- No
Offer Description
The goal of this project is to elucidate the influence of phospholipid bilayers and their models on interactions of biological peptides and related molecules with copper ions. This knowledge is required to better understand the processes involving copper signaling in health and disease. Biological compartments, relevant to copper signaling, including synapses, have very high surface to volume aspects, resulting in copper complexes present in the vicinity of the membrane. Still, their interactions are barely known. We will use exact physicochemical methods, to establish whether the phospholipid membrane may influence the biological chemistry of Cu2+/Cu+ ions. The obtained knowledge will contribute to designing innovative metallodrugs and therapies. The project will consist of nine tasks:
1. Peptide synthesis. Peptidic neuromodulators, Aβ species, NKB, NMC, hepcidin and other disulfide neuropeptides will be synthesized according to Fmoc strategy. Trp mutants and shorter model peptideswill be also obtained.
2. The effect of micellar environment on the Cu(II) binding affinity of neuromodulators. Equilibrium constants of Cu(II) complexes with neuromodulators will be determined in micellar systems.
3. Structural characterization of selected complex/micelle systems. This will be accomplished by NMR, including 19F spectra for Cu(II) complexes and isostructural Cu(II) replacements with diamagnetic Ni(II) or Pd(II).
4. Susceptibility of selected complex/micelle systems to competition from small hydrophilic ligands. The expected weakening of Cu(II) binding affinity of neuromodulators may make them susceptible to competition neurotransmitters, including glutamic acid, histidine, carnosine, taurine, dopamine, acetylcholine, noradrenaline, serotonin, and short histidine peptides.
5. Susceptibility of selected complex/micelle systems to reducing agents. Cu(II) complexes must be reduced to Cu(I) for hCtr1 transport. This will be tested using GSH, cysteine and ascorbate. The interference by small competitors and Zn2+ ions will also be studied.
6. The effect of micellar environment on the kinetics of exchange of Cu(II) ions between neuromodulators and other synaptic ligands. This will be studied by stopped-flow. Reactions faster than 2 ms will be approached by freeze quench kinetics coupled with EPR. Competition between Zn(II) and Cu(II)/Cu(I) ions will also be studied.
7. Synthesis of O-dodecylserine (ODS) and creation of Cu(II)-binding micelles. ODS will be synthesized for the first time, to serve as SDS-compatible analog of phosphatidylserine, a Cu(II) binding phospholipid. Its ability to form micelles will be tested, followed by studies of Cu(II) binding, competition with neuromodulators, redox and kinetic studies.
8. Interactions of Cu(II) complexes with negatively charged, neutral and Cu(II)-binding liposomes. Unilamellar liposomes will be prepared using phospholipids with distinct types of polar heads, followed by Trp fluorescence assays to test whether the interactions in liposomes correspond to micellar systems.
9. Cellular test of suitability of neuromodulators for copper delivery to cells. We developed an innovative protocol for “clean” exposure of HEK293 culture to Cu(II) complexes in a medium free environment, empowering the chemically selective interaction of these complexes with hCtr1. Promising complexes from previous tasks will be verified here.
The main focus of the PhD candidate will be on Tasks 1 and 7-9, with participation in the remaining ones.
Where to apply
Requirements
- Research Field
- Biological sciences
- Education Level
- Master Degree or equivalent
Hold a degree of Master of Science [Magister], Master of Engineering [Magister Inżynier], medical doctor or equivalent in the field of: exact sciences, natural sciences, medical sciences or related disciplines, granted by a Polish or foreign university; a person who does not possess the qualifications described above may take part in the competition, but must obtain the qualifications in question and provide the relevant documents before the start of the programme at the Doctoral School (i.e., 1st October 2025). Education at the Doctoral School begins on 1st October 2025.
Laboratory experience with organic synthesis, liposomes or cell culture will be a plus.
- Languages
- ENGLISH
- Level
- Excellent
- Research Field
- Biological sciences
Additional Information
- Scholarship amount (net, monthly, PLN): 1st year: 4000; 2nd year: 4000; 3rd year: 4 930,00; 4th year: 4 930,00;
- Possibility of renting a room in the Institute's hotel for the first 3 months at a special price (depending on rooms’ availability);
- Possibility to join a medical plan;
- Basic dental treatment;
- Research mini-grants for doctoral students (up to PLN 20000, a PhD student may apply for funds twice during his/her studies);
- Conference Grants (each PhD student can use up to PLN 12,000 to attend conferences during his/her studies);
- Surcharge for the multi sport card;
- Support in obtaining a temporary residence card (+ Reimbursement of TRC application expenses);
- Language courses (Polish, English, German, French, Spanish, Italian… (the offer of courses may change);
- Psychological care (up to 5 visits);
- Subsidy for the purchase of eyeglasses (once every two years);
- Free parking spot.
The recruitment process consists of two stages:
1. Selection of candidates by the Committee based on their previous achievements and academic performance presented in the documents submitted; for each position no more than 5 applicants who have achieved the highest scores, but no less than 60% of the maximum points, shall be qualified for the next stage;
2. An interview conducted by the Committee including in particular:
• a presentation delivered by the candidate containing the outcomes of his/her research (a Master’s thesis or other research work carried out by the candidate); the presentation must not last longer than 10 minutes;
• questions asked by the members of the Committee related to the presented project, the methods used and interpretation of the results obtained;
• questions asked by the members of the Committee related to the proposed PhD programme described in the recruitment announcement;
• questions related to the candidate’s motivation for scientific work.
Stage One
• learning outcomes (a scale of the evaluation: 0.0 ‒ 6.0 points);
• participation in a scientific project or an academic conference (a poster or oral presentation) (a scale of the evaluation: 0.0 ‒ 1.0 points);
• co-authorship of a research paper (depending on the role in the publication) (a scale of the evaluation: 0.0 ‒ 1.0 points);
• involvement in science club (a scale of the evaluation: 0.0 or 0.5 points);
• other achievements, e.g., awards, honors, scholarships, domestic and foreign internships, voluntary work, popularization of science (a scale of the evaluation: 0.0 ‒ 1.5 points).
Stage Two
• understanding of the project performed and methods used; the ability to interpret the results obtained; knowledge in the field related to the presented project (a scale of the evaluation: 0-10 points);
• knowledge in the field related to the proposed PhD programme described in the recruitment announcement (a scale of the evaluation: 0-10 points);
• form of presentation of the candidate’s results (a scale of the evaluation: 0-3 points).
- Website for additional job details
Work Location(s)
- Number of offers available
- 1
- Company/Institute
- Institute of Biochemistry and Biophysics Polish Academy of Sciences
- Country
- Poland
- City
- Warsaw
- Postal Code
- 02-106
- Street
- ul. Pawińskiego 5a
Contact
- City
- Warsaw
- Website
- Street
- ul. Pawińskiego 5a
- Postal Code
- 02-106
- PhDschool-recruitment@ibb.waw.pl